COVID19N-REAAD Anti-SARS-CoV-2 Qualitative Nucleocapsid Protein IgG ELISA Assay Kit

COVID19N-REAAD Anti-SARS-CoV-2 Qualitative Nucleocapsid Protein IgG ELISA was developed and manufactured by Restalyst

Size: 1×96 wells
Incubation Time: 1.5 Hours
Sample Type: Serum or Plasma
Species Sample: Human
Sample Size: 2 µL
Alternative Names: Serology, Coronavirus, COVID-19, Corona, Serological, COVID Antibody
For Research Use Only and not for use in diagnostic procedures.

Controls Included

Assay Principle

Recombinant SARS-CoV-2 nucleocapsid proteins are bound onto the microwells. Diluted serum specimens are added to antigen-coated wells and incubated. After incubation and washing, human antibodies targeting SARS-CoV-2 antigens remain bound to the well surface. Secondary antibody conjugated to horseradish peroxidase (HRP) targeting human IgG is then added to each well. After incubation, the ELISA wells are washed once again before a tetramethylbenzidine (TMB) substrate is added. As the assay determination is based on enzymatic reaction, the reaction can be stopped by the addition of 1N H2SO4, which will turn the blue coloration to yellow. The wells can then be read on any suitable spectrophotometer or microwell ELISA plate reader. It is always recommended to read the wells at 450 nm against a 620-630 nm reference filter to eliminate any possible causes of interference.

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