COVID-19 Nucleocapsid IgG Quantitative ELISA Assay Kit

COVID-19 Nucleocapsid IgG Quantitative ELISA Assay Kit was developed and manufactured in the US

Size: 1×96 wells
Sensitivity: 0.17 U/mL
Dynamic Range: 0.17-200 U/mL
Incubation Time: 1.5 Hours
Sample Type: Serum
Species Sample: Human
Sample Size: 10 µL
Alternative Names: Serology, Coronavirus, COVID-19, Corona, Serological
RUO, CE-Marked

Controls Included

Cross Reactivity

Panels were studied with a minimum of eight confirmed disease state samples with this serology assay. No interference was observed for the following disease or infectious agents:

• Anti-influenza A
• Anti-influenza B
• Hepatitis C (HCV)
• Antinuclear Antibodies (ANA)
• Respiratory Syncytial (RSV)

Assay Principle

The COVID-19 Nucleocapsid IgG Quantitative ELISA Assay kit is designed, developed, and produced for the quantitative measurement of the human anti-COVID-19 IgG antibody in serum. This assay utilizes the microplate based enzyme immunoassay technique.

Assay calibrators, controls, and 1:100 diluted human serum samples are added to the microtiter wells of a microplate that was coated with COVID-19 recombinant full length nucleocapsid protein. After the first incubation period, the unbound protein matrix is removed with a subsequent washing step. A horseradish peroxidase (HRP) labeled polyclonal goat anti-human IgG tracer antibody is added to each well. After an incubation period, an immunocomplex of “COVID-19 recombinant antigen – human anti-COVID-19 IgG antibody – HRP labeled anti-human IgG tracer antibody” is formed if there is specific coronavirus IgG antibody present in the tested specimen. The unbound tracer antibody is removed by the subsequent washing step. HRP-labeled anti-hIgG tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to the anti-COVID-19 IgG on the wall of the microtiter well is proportional to the amount of the anti-COVID-19 IgG antibody level in the tested specimen.

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