Anti-SARS-CoV-2 Spike Protein IgG Qualitative ELISA

The Anti-SARS-CoV-2 Spike Protein IgG Qualitative ELISA is For Research Use Only

Size: 12×8 wells
Sensitivity: 800 pg/ml
Standard Range: 0 pg/ml – 7500 pg/ml
Incubation Time: 2.5 hours
Sample Type: Respiratory Specimens, serum, plasma, cell culture supernate
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV

Assay Principle

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Coronavirus present in the sample are bound by the antibodies. HRP labeled anti-Human IgG is added and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Coronavirus in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Sample Preparation and Storage
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.
Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided. If samples are to be used for several assays, initially aliquot samples and keep at – 20°C.
Cell Culture Supernates – Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃ or lower temperature. Avoid repeated freeze-thaw cycles. If the use of original supernate sample or low dilution (<5 fold)="" are="" necessary="" due="" to="" the="" expected="" low="" concentration="" of="" antigen="" supernates="" need="" be="" adjust="" to="" neutral="" ph="" condition="" before="" assay.="" should="" you="" desire="" to="" inactivate="" the="" virus,="" use="" a="" (5x)="" lysis="" buffer="" (optional,="" not="" provided).="" add="" 1/5="" volume="" of="" (5x)="" lysis="" buffer="" to="" sample="" (i.e.="" add="" 50="" ul="" (5x)="" lysis="" buffer="" to="" 200="" ul="" sample).="" vortex="" well.="" note:="" the="" sample="" should="" be="" diluted="" to="" within="" the="" working="" range="" of="" the="" assay="" in="" 1x="" assay="" diluent.="" the="" exact="" dilution="" must="" be="" determined="" based="" on="" the="" concentration="" of="" specific="" target="" in="" individual="" samples.="" respiratory="" sample="" -="" centrifuge="" samples="" for="" 20="" minutes="" at="" 10000="" x="" g="" at="" 2-8°c.="" collect="" supernatant="" and="" carry="" out="" the="" assay="" immediately.="">

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