14,15 EET DHET ELISA

The 14,15 EET DHET ELISA is For Research Use Only
Product manufactured in the USA
Size: 1×96 wells
Sensitivity: 0.01 ng/ml
Dynamic Range: 0.01 – 1000 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma, Tissue, Biological Fluids
Sample Size: 100 µl

Storage and Stability
The 14,15 EET/DHET ELISA will obtain optimal results if all of the components are stored at the proper temperature prior to use. Items should be stored at the designated temperatures upon receipt. All components are stored below -20°C and should not be re-frozen and thawed more than necessary.

Assay Background

It is well known that arachidonic acid (AA) will be converted to EET by P450 arachidonic acid epoxygenase (AA epoxygenase) and EET will be converted to DHET by soluble epoxide hydrolase (sEH) in vivo.  Cytochrome P450 2J2 (CYP2J2) is a predominant human AA epoxygenase that produces all four EETs. In human carcinoma cells, rAAV-mediated over expression of CYP2J2 resulted in a marked increase in 14,15-DHET level in cell plasma, whereas rAAV-anti2J2-mediated silence of CYP2J2 expression significantly decreased its production (1).  This ELISA Assay kit can be used to measure EET levels in cultured cells which express sEH (1).  

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