MutaGEL r-Calcitonin PCR Assay Kit

MutaGEL r-Calcitonin PCR Assay manufactured in Germany by Immundiagnostik

Size: 24 Samples
Method: PCR (RFLP)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
For Research Use Only

Controls Included

Storage and Stability
Store at ≤ -18°C. The reagents are stable in the unopened micro tubes until the expiration date indicated (see print on the package). Do not thaw out the content of the “CTR positive control DNA” for more than two times. If necessary, make suitable aliquots. Before use: Spin tubes briefly before opening (contents may become dispersed during shipment).

Assay Principle

The MutaGEL® r-Calcitonin kit contains a set of primer for amplification of the specific sequence within the human calcitonin receptor gene CTR. Amplificates of varying genotypes (C→T mutation, Pro463Leu polymorphism) is characterized by subsequent specific restriction enzyme digestion.  The variant (C, protective) possesses a restriction site for the endonuclease whereas the other variant (T, pathogen) does not. The amplified products obtained from the protective DNA-variant will, therefore, be cut into two fragments, whereas the pathogen DNA-variant will not be cut. The identification of the present genotype is done by analysis of present DNA-fragments by gel electrophoretic methods (Dr. Essrich, Biologisches Labor, Denzlingen).

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