MutaGEL Oxidative Stress I PCR Assay

MutaGEL Oxidative Stress I PCR Assay manufactured in Germany by Immundiagnostik

Size: 24 Samples
Method: PCR (RFLP)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
For Research Use Only

Controls Included

Assay Principle

With the MutaGEL OxiStress I PCR Assay two DNA regions specific for the critical gene parts of eNOS and NAD(P)H Oxidase are amplified in parallel reactions. The amplification products are then treated with a mix of restriction enzymes, which are sensitive for the “negative” sequence variant of each gene – in parallel as well – such that in the following gel the genotype of the probe can be recognised for both polymorphisms from the length of the resulting restriction fragments (RFLP method).

Sample Preparation

• Extract total genomic DNA (for example from 200 µl of whole blood) using a commercially available DNA isolation kit.
• Start immediately with the amplification procedure or store the extracted DNA at < -18°C.

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Restrictions

The PCR results for all positive controls in DNA fragments of indicated length and for samples at least in the amplification product indicated length. If this is not the case, the sample must be tested a second time or the complete analysis must be repeated with freshly isolated DNA. If there are no positive control DNA fragments present, the amplification was incorrect and the chosen PCR conditions have to been proven/ corrected.