3-HAA rabbit Polyclonal Antibody

The 3-HAA rabbit Polyclonal Antibody is For Research Use Only

When this antibody was tested by a competitive ELISA, it did not show any significant cross reactivity with Anthranilic acid or quinolinic acid conjugates.

Synonyms: Anti-Conjugated 3-Hydroxyanthranilic Acid Antibody, Anti-3-Hydroxy-Anthranilic acid rabbit polyclonal antibody, 2-Amino-3-hydroxybenzoic acid rabbit polyclonal antibody 3-OH-Anthranilic acid rabbit polyclonal antibody, 3-hydroxanthranilate rabbit polyclonal antibody, 3-OHAA rabbit polyclonal antibody, 3-HAA rabbit polyclonal antibody
Immunogen:  Conjugated 3-Hydroxyanthranilic acid
Size:  50µl
Storage:  4°C
Storage buffer: Before use, vial should be resuspended in 50 µL of ultrapure water. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles

Affinity & Specificity:
Competitive ELISA demonstrates that low amounts of 3-HAA conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Anthranilic and Quinolinic acid conjugates do not affect reaction (high specificity).

Protocols

Immunocytochemistry(ICC): Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections
Immunohistochemistry (IHC): Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate.
Immunohistofluorescence (IHF): Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate.

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