Deoxynivalenol & Zearalenon Immunoaffinity Column

The Deoxynivalenol & Zearalenon Immunoaffinity Column is For Research Use Only

Assay Instructions

This instruction of Deoxynivalenol (DON) and Zearalenon (ZON) determination in food and feed focuses on the enrichment step of extract using immunoaffinity column (IAC) and quantification with HPLC. Accepted laboratory extraction methods could be maintained. Full performance of the IAC column is given if pronounced criteria regarding organic solvent tolerance, elution process of analyte and working range of column is followed. Many pretreatment methods of DON and ZON determination in food and feed show low sensitivity because of interfering substances if problematic matrices are applied.

This method of content determination of DON and ZON combines the high selectivity of an immunoaffinity column (IAC) with its potential to concentrate elute and additional step of purification by HPLC column.

Please notice that this instruction focuses on the handling with the IAC column. For the commodity extraction step a literature method is given. The given apparatus (e.g. HPLC system) might serve as example among other possibilities.

Size: 3ml, 10 columns
Extraction (Literature method given)
Assuming that 25g ground corn sample are extracted by a total of 100ml acetonitrile/water (75/25, v/v), as reported by Krska et al. If organic solvent proportion is varied the dilution of extract with PBS should be adapted accordingly in the enrichment step. On the other hand, if proportion of sample quantity and volume of extraction solvent is altered, calculation of gram equivalents must be corrected.

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Product Developed and Manufactured in Germany by BioTeZ Berlin-Buch GmbH