Aflatoxin M1 Immunoaffinity Column

The Aflatoxin M1 Immunoaffinity Column is For Research Use Only

Assay Instructions

This Eagle Biosciences Aflatoxin M1 Immunoaffinity Column is for the clean-up of milk and milk products containing Aflatoxin M1. Immunoaffinity chromatography stands for an efficient enrichment process of aflatoxins with concomitant effective separation from matrix components. The eluate is best suited for determination by HPLC. By varying washing process, that means exchange PBS buffer with 0.2M ammonium acetate solution, GC can also be applied.

Sample Preparation of Milk

According to the method of Shundo et al.1 the milk to be tested is first centrifuged by 1540g for 15 minutes. The cream is then separated using e.g. a separation funnel. If separation is incomplete, the milk sample should be filtered to avoid that remaining fatty particles block the column in next step below.

Cross Reactivity

Aflatoxin M1 (AFM1): 100%
Aflatoxin B1 (AFB1): 98%
Aflatoxin B2 (AFB2): 99%
Aflatoxin G1 (AFG1): 50%
Aflatoxin G2 (AFG2): 98%

Learn more about our Immunoaffinity Columns at Eagle Biosciences’ Product Highlights page dedicated to Immunoaffinity here:

Immunoaffinity Columns: Product Highlights

Product Developed and Manufactured in Germany by BioTeZ Berlin-Buch GmbH

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