Soluble Leptin Receptor ELISA

The Soluble Leptin Receptor ELISA is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.0385 ng/mL
Standard Range: 0.0385 – 150 ng/mL
Incubation Time: 5 hours
Sample Type: Serum or Plasma
Sample Size: 30 µL
Controls Included

Product is manufactured by Mediagnost

Assay Background

The adipokine leptin realizes signal transduction via four different leptin receptor (LEP-R) isoforms. The amount of functionally active LEP-R, however, is affected by constitutive shedding of the extracellular domain. The product of the cleavage process, the so-called soluble leptin receptor (sLEP-R, soluble Leptin receptor), is the main binding protein for leptin in human blood and modulates its bioavailability. Concentrations of sLEP-R are differentially regulated in metabolic disorders, such as type 1 diabetes mellitus or obesity and can therefore enhance or reduce leptin sensitivity. Lipotoxicity and apoptosis increase LEP-R cleavage via ADAM10-dependent mechanisms. In contrast, although increased sLEP-R concentrations seem directly to inhibit leptin effects, reduced amounts of sLEP-R may reflect decreased membrane expression of LEP-R. These findings, in part, explain alterations of leptin sensitivity that are associated with changes in serum sLEP-R concentrations seen in metabolic disorders.

Sample stability
In firmly closable sample vials:
• Storage at Room Temperature 20-25°C: max. 2 days
• Storage at 4°C: max. 3 days
• Storage at -20°C: 2 years
• Freeze/Thaw cycles: max. 3
Freeze-Thaw cycles should be minimized. Up to 3 cycles showed no effect on the measured sLEP-R concentration.
Use standard venipuncture for the blood sampling. Haemolytic reactions have to be avoided.

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