Nitrotyrosine ELISA Assay Kit

The Nitrotyrosine ELISA Assay Kit is For Research Use Only

Sensitivity: 50 nM
Dynamic Range: 62.5 – 8000 nM
Incubation Time: 1 hour
Sample Type: Cell lysates, Plasma, Serum, Urine
Sample Size: 50 μL

Product manufactured in Canada by StressMarq.

Assay Background

Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present. Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.

Storage and Stability
This kit will perform as specified if the components are stored as directed and used before the expiration date indicated on the outside of the box. All reagents are stable as supplied at 4°C. For optimum storage, the Standard should be aliquotted into smaller portions and then stored appropriately. Avoid repeated freeze/thaw cycles (10 µL of Standard can prepare a triplicate standard curve.

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