FluoBolt-Noggin Fluorescence Immunoassay

The FluoBolt Noggin Fluorescence Immunoassay is manufactured in Austria by Fianostics GmbH

Method: Metal Enhanced Direct Sandwich Fluorescence Immunoassay
Size: 1×96 wells
Sensitivity: LOD <0 pmol/l + 3 SD): 10 pmol/l; LLOQ: 5 pmol/l
Dynamic Range: 0-250 pmol/l
Incubation Time: Overnight
Sample Type: Serum, Plasma
Sample Size: 20 µl
For Research Use Only

This platform is fully compatible to standard laboratory methodology using 96 well microtiter plate format and assays based on this technology can be run on any standard fluorescence microplate reader.

This FluoBolt-Noggin Fluorescence Immunoassay is regularly delivered standard with AlexaFluor680 labeled detection-antibody. If you wish to use a different dye, the following options are available

• FITC labeled (FIA-1701-F)
• Cy3 labeled (FIA-1701-C3)
• Cy5 labeled (FIA-1701-C5)

Conversion factor: 1 pg/ml = 0.015 pmol/l (MW: 66 kD)

Assay Background

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Human Noggin cDNA encodes a 232 amino acid (aa) precursor protein; cleavage of a 19 aa signal peptide generates the 213 aa mature protein which contains an N-terminal acidic region, a central basic heparin-binding segment and a C-terminal cysteine-knot structure. Secreted Noggin probably remains close to the cell surface due to its binding of heparin containing proteoglycans. Noggin is very highly conserved among vertebrates.

Noggin predominantly binds BMP-4 and BMP-2 and antagonizes their bioactivities by preventing binding to both type I and type II receptors. During embryogenesis Noggin is a crucial factor for regulation of various developmental processes like formation of neural tubes, cardiomyocyte growth and patterning as well as skeletal development where Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints. Mutations within the cysteine-knot region of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin.
NOGGIN has been linked to:

• Bone Tumors
• Ankylosing Spondylitis
• Non Alcoholic Fatty Liver Disease (NAFLD)
• Pulmonary Arterial Hypertension (PAH)

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