SARS-CoV-2 Spike RBD Antigen Quantitative ELISA

The SARS-CoV-2 Spike RBD Antigen Quantitative ELISA is For Research Use Only

Size: 12×8 wells
Standard Range: 0-4000 ng/ml
Incubation Time: 3 hours 20 minutes
Sample Type: Serum
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19

Assay Principle

The method employs sandwich ELISA technique. Monoclonal antibody specific for SARS-CoV-2 (2019-nCoV) Antibodies to Spike RBD is pre-coated onto microwells. Samples and standards are pipetted into microwells and SARS-CoV-2 (2019-nCoV) Spike RBD Antigen present in the sample are bound by the immobilized antibody. After incubation the wells are washed and followed by addition of HRP-conjugated Detection antiSARS-CoV-2 (2019-nCoV) Spike RBD antibody into each well and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of SARS-CoV-2 (2019-nCoV) Spike RBD in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Sample Preparation and Storage
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.
Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Repeated freezing and thawing should be avoided. If samples are to be used for several assays, initially aliquot samples and keep at -20°C.
Samples should be diluted 1:1000 (v/v) for optimal recovery, (for example 1 ul sample + 999 ul sample diluent) prior to assay. In cases where matrix interferences is under or over observed, the samples may be diluted with Sample Diluent accordingly.
The samples may be kept at 2 – 8°C for up to three days. For long-term storage please store at -20°C.
Note: Grossly hemolyzed samples are not suitable for use in this assay

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