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商品详细EagleBio/SARS-CoV-2棘突蛋白抗原定量ELISA/100µL/KBVH015-10
EagleBio/SARS-CoV-2棘突蛋白抗原定量ELISA/100µL/KBVH015-10
EagleBio/SARS-CoV-2棘突蛋白抗原定量ELISA/100µL/KBVH015-10
商品编号: KBVH015-10
品牌: EagleBio
市场价: ¥18400.00
美元价: 9200.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

SARS-CoV-2 Spike Protein Antigen Quantitative ELISA

The SARS-CoV-2 Spike Protein Antigen Quantitative ELISA is For Research Use Only

Size: 12×8 wells
Sensitivity: 6.8 ng/ml
Standard Range: 0- 500 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, cell culture supernatant and plasma
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19


Assay Principle

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human SARS-CoV-2 (Covid-19) spike proteins present in the sample are bound by the capture antibodies. After incubation the wells are washed and followed by HRP- conjugated Detection Antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human SARS-CoV-2 (Covid-19) spike proteins in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.


Sample Preparation and Storage
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.
Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided.
Samples should be diluted 1:5000 (v/v) for optimal recovery, (for example 1 ul sample + 4999 ul sample diluent) prior to assay. In cases where matrix interferences is under or over observed, the samples may be diluted with Sample Diluent accordingly. The samples may be kept at 2 – 8°C for up to three days. For long-term storage please store at -20°C.
Note: Grossly hemolyzed samples are not suitable for use in this assay
Cell Culture Supernates – Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃ or lower temperature. Avoid repeated freeze-thaw cycles. If the use of original supernate sample or low dilution (<5 fold)="" are="" necessary="" due="" to="" the="" expected="" low="" concentration="" of="" antigen="" supernates="" need="" be="" adjust="" to="" neutral="" ph="" condition="" before="" assay.="" note:="" the="" sample="" should="" be="" diluted="" to="" within="" the="" working="" range="" of="" the="" assay="" in="" 1x="" assay="" diluent.="" the="" exact="" dilution="" must="" be="" determined="" based="" on="" the="" concentration="" of="" specific="" target="" in="" individual="" samples.="">


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品牌介绍
Eagle Biosciences已迅速成为全球研究和临床社区免疫测定,抗体和蛋白质的先进供应商。我们的员工在研究和诊断行业拥有超过25年的经验,在商业化和分销方面拥有超过10年的经验。除了分销来自20多个欧洲组织的产品外,我们还提供EagleBio开发的产品。通过所有这些产品,我们将主要专注于提供有助于医学研究和临床实验室的深奥和创新产品eaglebio新产品介绍FluoBolt-Noggin Fluorescence Immunoassay荧光免疫法SKU:FIA-1702-A6-1分类:骨代谢氟硼酸盐- Noggin Fluorescence Immunoassay是用于定量测定人血清或血浆中NOGGIN的方法。FluoBolt™-Noggin荧光免疫分析与骨肿瘤、强直性脊柱炎和肺动脉高压等疾病有关。