MutaGEL Prothrombin PCR Assay

MutaGEL Prothrombin PCR Assay manufactured in Germany by Immundiagnostik

Size: 24 Samples
Method: PCR (RFLP)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
For Research Use Only
Controls Included

Storage and Stability
Storage at < -18°c.="" reagents="" are="" stable="" in="" unopened="" micro="" tubes="" until="" the="" expiration="" date="" indicated.="" in="" general,="" avoid="" several="" thawing/freezing="" –="" cycles="" for="" the="" reagents.="" before="" use:="" spin="" tubes="" briefly="" before="" opening="" (contents="" may="" become="" dispersed="" during="" shipment).="">Restrictions
The PCR results for positive control in DNA fragments of indicated length and for the samples at least one of both amplification products must be detectable. If this is not the case, the sample must be tested a second time or the complete analysis must be repeated with freshly isolated DNA. If there are no positive control DNA fragments present, the amplification was incorrect and the chosen PCR conditions have to been proven/ corrected.

Assay Principle

The MutaGEL® Prothrombin Factor II PCR Assay Kit contains a set of primers which amplify a specific sequence within the human factor II gene. The amplified product obtained from a wild-type DNA will not be cut by the restriction enzyme included in this kit, whereas the fragment obtained from DNA homozygous for the factor II G20210A mutation will be cut. Amplification products and their cut fragments are analyzed through gel electrophoresis.

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